Integrin-Associated Complexes Form Hierarchically with Variable Stoichiometry in Nascent Adhesions
نویسندگان
چکیده
منابع مشابه
Integrin-Associated Complexes Form Hierarchically with Variable Stoichiometry in Nascent Adhesions
BACKGROUND A complex network of putative molecular interactions underlies the architecture and function of cell-matrix adhesions. Most of these interactions are implicated from coimmunoprecipitation studies using expressed components, but few have been demonstrated or characterized functionally in living cells. RESULTS We introduce fluorescence fluctuation methods to determine, at high spatia...
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We describe a method to detect molecular complexes and measure their stoichiometry in living cells from simultaneous fluctuations of the fluorescence intensity in two image channels, each detecting a different kind of protein. The number and brightness (N&B) analysis, namely, the use of the ratio between the variance and the average intensity to obtain the brightness of molecules, is extended t...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2014
ISSN: 0960-9822
DOI: 10.1016/j.cub.2014.07.011